Genetic and adaptive variation in beech (Fagus sylvatica): Which factors influence their spatial distribution patterns?

 

Previous project phase (2014 - 2017)

4th Phase - Research without DFG-funding

 

Scientific investigators:

Dr. Markus Müller

(Uni Göttingen)

Aims

1. Identification of probably adaptive genes

2. Analysis of the genetic diversity within these candidate genes for the adaptive relevant traits drought resistance and bud burst, since the exploratories represent a precipitation and a temperature gradient

3. Investigation of the adaptability of beech seedlings in reciprocal translocation experiments with the aim to associate genetic variation with phenotypic traits (in a possible follow up project)

 

Hypotheses

H1. We expect a higher differentiation in adaptive genes in comparison to “neutral” genetic variation.

H2. We expect the local seedling to be best adapted in comparison to seedlings from other exploratories.

H3. Furthermore, the influence of different forest management intensities on probably adaptive variation in candidate genes will be investigated.

 

Methods

1. Transcriptome analysis by means of next generation sequencing as well as the utilization of candidate genes already used in former studies on beech and oak (Quercus spp.)

2. Analysis of selected SNPs (Single Nucleotide Polymorphisms) within the candidate genes

3. Observation of phenotypic traits, for example survival, bud burst and growth

 

Previous project phase (2011 - 2014)

 

Scientific investigators:

Prof. Dr. Reiner Finkeldey

Markus Müller

(Uni Göttingen)

Aims

1.    Identification of probably adaptive genes

2.    Analysis of the genetic diversity within these candidate genes for the adaptive relevant traits drought resistance and bud burst, since the exploratories represent a precipitation and a temperature gradient

3.    Investigation of the adaptability of beech seedlings in reciprocal translocation experiments with the aim to associate genetic variation with phenotypic traits (in a possible follow up project)

 

Hypotheses

H1.    We expect a higher differentiation in adaptive genes in comparison to “neutral” genetic variation.
H2.    We expect the local seedling to be best adapted in comparison to seedlings from other exploratories.
H3.    Furthermore, the influence of different forest management intensities on probably adaptive variation in candidate genes will be investigated.

 

Methods

1.    Transcriptome analysis by means of next generation sequencing as well as the utilization of candidate genes already used in former studies on beech and oak (Quercus spp.)

2
.    Analysis of selected SNPs (Single Nucleotide Polymorphisms) within the candidate genes

3.   
Observation of phenotypic traits, for example survival, bud burst and growth

 

Previous project (will be completed in 2011): BEECHGEN

Analysis of neutral genetic diversity on different spatial scales (with the help of microsatellite and AFLP marker) to investigate the influence of different forest management intensities on the genetic structure of beech