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Little is known about the diversity and distribution of methylotrophs. The objective of this project is to analyse the genetic diversity, biogeography, and functional role of phyllosphere methylotrophs in dependence of inhabited plant species, land-use type and geographic location. Pink-pigmented facultative methylotrophs (PPFM) add up to 79% of the total number of the heterotrophs found in the phyllosphere. That is why this subgroup of the methylotrophs will be the main focus of our project.


1. The diversity of methylotrophic bacteria is dependent on the land use intensity, the host plant species and the spatial distribution.

2. The production of plant growth promoting substances by methylotrophic bacteria is dependent on the microbial species composition and the land use intensity.

3. The genetic variability within a species correlates with land use intensity and / or geographic distance.


Leaf samples of Trifolium repens (white clover), Cerastium holosteoides (common mouse-ear) und Plantago lanceolata (ribwort plantain) were collected from sampling sites with different land-use types. To investigate the concentration of the PPFM per g leaf weight, the bacteria isolated from the leaf samples were cultivated on selective medium supplemented with methanol. For representative isolates, the 16S rRNA genes were sequenced and the resulting sequence information was used for phylogenetic analyses. Intraspecies diversity was studied by sequencing the mxaF-gene of selected isolates. The mxaF-gene codes for the ?-subunit of the methanoldehydrogenase which is present in all methylotrophs. In the near future the composition of the phyllosphere methylotrophs inhabiting the different leaf samples will be studied by fingerprinting methods (DGGE).


Erste Ergebnisse sind:

  • Landnutzungstypen und Pflanzenarten haben einen signifikanten Einfluss aufdie Konzentration von methylotrophen Bakterien auf Blattoberflächen.
  • Fünf phylogenetisch verschiedene Methylobacterium Gruppen einschl. drei neuer Arten wurden gefunden.
  • Die Artenzusammensetzung der methylotrophen Bakterien ist nicht durch die Landnutzung beeinflusst.

Doc
Isolierung von Williamsia phyllosphaerae sp. nov von der Oberfläche von Trifolium repens
Kämpfer P., Wellner S., Lohse K., Lodders N., Martin K. (2011): Williamsia phyllosphaerae sp. nov, isolated from the surface of Trifolium repens. International Journal of Systematic and Evolutionary Microbiology 61, 2758 – 2762. doi: 10.1099/ijs.0.029322-0
More information:  doi.org
Doc
Isolierung von Duganella phyllosphaerae sp. nov. von der Blattoberfläche von Trifolium repens und Antrag, Duganella violaceinigra in eine neue Gattung als Pseudoduganella violceinigra gen. nov., comb. nov. einzuordnen
Kampfer P., Wellner S., Lohse K., Martin K., Lodders N. (2012) : Duganella phyllosphaerae sp. nov., isolated from the leaf surface of Trifolium repens and proposal to reclassify Duganella violaceinigra into a novel genus as Pseudoduganella violceinigra gen. nov., comb. nov. Systematic and Applied Microbiology 35 (1), 19-23. doi: 10.1016/j.syapm.2011.10.003.
More information:  doi.org
Doc
Diversity and biogeography of selected phyllosphere bacteria with special emphasis on Methylobacterium spp.
Diversität und Biogeographie von ausgewählten Bakterien der Phyllosphäre mit besonderem Schwerpunkt auf Methylobacterium spp.
Wellner S. A. (2011):Diversity and biogeography of selected phyllosphere bacteria with special emphasis on Methylobacterium spp. Dissertation, University Giessen
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Methylobacterium cerastii sp. nov., eine neue Art, welche von der Blattoberfläche von Cerastium holosteoides isoliert wurde
Wellner S. A., Lodders N., Kämpfer P. (2012): Methylobacterium cerastii sp. nov., a novel species isolated from the leaf surface of Cerastium holosteoides. International Journal of Systematic and Evolutionary Microbiology 62, 917–924, doi: 10.1099/ijs.0.030767-0
More information:  doi.org
Doc
Wellner S., Lodders N., Glaeser S. P., Kämpfer P. (2013): Methylobacterium trifolii sp. nov. and Methylobacterium thuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces. International Journal of Systematic and Evolutionary Microbiology 63, 2690–2699. doi: 10.1099/ijs.0.047787-0
More information:  doi.org
Doc
Diversität und Biogeographie von ausgewählten Bakterien der Phyllosphäre mit besonderem Schwerpunkt auf Methylobacterium spp.
Wellner S., Lodders N., Kämpfer P. (2011): Diversity and biogeography of selected phyllosphere bacteria with special emphasis on Methylobacterium spp. Systematic and Applied Microbiology 34(8), 621–630. doi: 10.1016/j.syapm.2011.08.005
More information:  doi.org

Scientific assistants

Prof. Dr. Peter Kämpfer
Project manager
Prof. Dr. Peter Kämpfer
Dr. Nicole Lodders
Project manager
Dr. Nicole Lodders
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