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Picture: The collage contains the logo of the König Research Museum and four photos. Photo 1 shows a portrait of Doctor Jan Struwe in black and white. Photo 2 shows a malaise trap in a garden next to a tree, which is used to catch flying insects. Approximately one metre above the ground, a kind of tent made of white fabric is attached to poles. Insects that fly under the tent are directed to a plastic bottle containing a preservative liquid. The bottle is located at the top next to the tent in a holder that is also mounted on poles. A solar panel is attached below the holder to power automatic separation processes of the samples. Photo 3 shows in a screenshot against a black background the signals of isolated D N A pieces of a sample shown in white. Photo 4 shows a fly photographed from above. Below the fly, a diagram is mounted that shows the base sequence of a gene section line by line on a white background. The four bases are shown in different colours.

The amount of different insect species is overwhelming and can only be guessed. Even if we reduce the investigated area and only look upon species that are present in Germany the diversity seems infinite. The time and number of experts needed to identify all these different species exceeds all reasonable numbers and can easily be called uneconomical. This identification process needs to be accelerated.Right now we can only look at restricted sets of species due to time and cost limitations. As we are missing a lot of information by this we need to capture a broad range of biodiversity, the ability to deal with known and unknown species and improve the cost efficiency by parallel processing of multiple samples. This will improve biomonitoring drastically.To achieve this improvement the identification process will focus on species specific genetic information.


The goals of the DNA Barcoding Project are:

  • shorten the time for species identification
  • improve the cost efficiency
  • deal with high species numbers of known and unknown species
  • independence of the species live stages

Doc
Struwe J.-F. (2020): Development of workflows for metabarcoding of mass-samples: A case study on Diptera. Dissertation, University Bonn
More information:  bonndoc.ulb.uni-bonn.de

Project in other funding periods

Picture: The collage contains the logo of the König Research Museum and four photos. Photo 1 shows a portrait of Doctor Jan Struwe in black and white. Photo 2 shows a malaise trap in a garden next to a tree, which is used to catch flying insects. Approximately one metre above the ground, a kind of tent made of white fabric is attached to poles. Insects that fly under the tent are directed to a plastic bottle containing a preservative liquid. The bottle is located at the top next to the tent in a holder that is also mounted on poles. A solar panel is attached below the holder to power automatic separation processes of the samples. Photo 3 shows in a screenshot against a black background the signals of isolated D N A pieces of a sample shown in white. Photo 4 shows a fly photographed from above. Below the fly, a diagram is mounted that shows the base sequence of a gene section line by line on a white background. The four bases are shown in different colours.
Barcoding Diptera (Contributing project)
#Animals  #2014 – 2017  

Scientific assistants

Prof. Dr. Wolfgang Weisser
Project manager
Prof. Dr. Wolfgang Weisser
Technische Universität München (TUM)
Prof. Dr. Wolfgang J. Wägele
Project manager
Prof. Dr. Wolfgang J. Wägele
Zoologisches Forschungsmuseum Alexander Koenig
Jan-Frederic Struwe
Employee
Jan-Frederic Struwe
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