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  1. Diversity of fungal peroxidases is larger in sites also rich in plant taxa.
  2. Diversity of fungal peroxidases is larger in forest sites than in agricultural sites.
  3. There is a continuum of peroxidases in soil and wood.

In this research project, we particularly address one ecological group of basidiomycetes, the litter decomposers, at the level of genes and proteins. Central to this endeavour are extracellular peroxidases, which are key enzymes in the degradation of lignin and humic substances. Beyond their ecological importance, these enzymes are of interest for biotechnological applications as they also catalyze pharmaceutically relevant reactions and oxidize pollutants. Peroxidases of soil and litter are detected on the DNA, RNA and enzyme protein level, and later will be characterized for their molecular and catalytical parameters.


Currently, participants of FUPERS work at optimizing molecular biological and protein chemical methods (e.g. design of peroxidase primers, enrichment of enzymes, detection in soil extracts), placing and processing of litter bags in Hainich, and the analysis of basidiomycete diversity based on specific primers. Microcosm experiments in the lab with Hainich-derived fungal strains will allow conclusions on the abundance of ligninolytic peroxidases and peroxygenases. The goal is to identify key players of lignin degradation to later detect them in the field (in soil and litter samples).


Doc
Kellner H., Luis P., Pecyna M. J., Barbi F., Kapturska D., Krüger D., Zak D. R., Marmeisse R., Vandenbol M., Hofrichter M. (2014): Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils. PLoS ONE 9(4): e95557. doi: 10.1371/journal.pone.0095557
More information:  doi.org
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Diversitäts-Bestimmungen in Umweltsequenzen sind stark abhängig von qualitativ hochwertigen Alignmentdaten von ITS- und neuen LSU-Primern für Basidiomyceten
Krüger D., Kapturska D., Fischer C., Daniel R., Wubet T. (2012): Diversity Measures in Environmental Sequences Are Highly Dependent on Alignment Quality—Data from ITS and New LSU Primers Targeting Basidiomycetes. PLoS ONE 7(2): e32139. doi:10.1371/journal.pone.0032139"
Doc
Muster des Ligninabbaus und der Sekretion oxidativer Enzyme durch verschiedene Holz- und Streubewohnende Basidiomyceten und Ascomyceten auf Buchenholz
Liers C., Arnstadt T., Ullrich R., Hofrichter M. (2011): Patterns of lignin degradationand oxidative enzyme secretion by differentwood- and litter-colonizing basidiomycetes and ascomycetes grown on beech-wood. FEMS Microbiology Ecology 78 (1), 91–102. doi: 10.1111/j.1574-6941.2011.01144.x
More information:  doi.org
Doc
Molekularbiologische Charakterisierung von Häm-Thiolat- und DyP-type-Peroxidasen ausgewählter Basidiomyceten
Pecyna M. (2016): Molecular biological charaterization of heme-thiolate and DyP-type peroxidases of selected basidiomycetes. Dissertation, TU Dresden
More information:  www.qucosa.de
Doc
Aufklärung des Genes für ein Peroxidase-Enzym aus dem Ständerpilz Südlicher Ackerling
Pecyna M.J., Ullrich R., Clemens A., Schubert R., Scheibner K., Hofrichter M. (2009): Molecular characterization of aromatic peroxygenase from Agrocybe aegerita. Applied Microbiology and Biotechnology, Vol 84 Nr. 5, S. 885-897
More information:  doi.org
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Sind Korrelationen zwischen der Artengemeinschaft von holzzersetzenden Pilzen, Holzeigenschaften und Holzenzymen stabil entlang eines großskalierten Landschaftsgradienten?
Purahong W., Arnstadt T., Kahl T., Bauhus J., Kellner H., Hofrichter M., Krüger D., Buscot F., Hoppe B. (2016): Are correlations between deadwood fungal community structure, wood physico-chemical properties and lignin-modifying enzymes stable across different geographical regions? Fungal Ecology 22, 98–105. doi: 10.1016/j.funeco.2016.01.002
More information:  doi.org
Doc
Einfluss verschiedener Waldbewirtschaftungssysteme auf die Abbaurate der Laubstreu, Nährstoffdynamik und Aktivität ligninzersetzender Enzyme.
Purahong W., Kapturska D., Pecyna M. J., Schulz E., Schloter M., Buscot F., Hofrichter M., Krüger D. (2014): Influence of Different Forest System Management Practices on Leaf Litter Decomposition Rates, Nutrient Dynamics and the Activity of Ligninolytic Enzymes: A Case Study from Central European Forests. PLoS ONE 9(4): e93700. doi: 10.1371/journal.pone.0093700
More information:  doi.org

Scientific assistants

Prof. Dr. Martin Hofrichter
Project manager
Prof. Dr. Martin Hofrichter
TU Dresden, Internationales Hochschulinstitut Zittau
Dr. Dirk Krüger
Project manager
Dr. Dirk Krüger
Danuta Kapturska
Employee
Danuta Kapturska
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