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Picture: The collage contains six photographs of steps in molecular analyses in a laboratory, performed by a scientist. Photo 1 shows a sample standardization device. Photo 2 shows the scientist working on a polymerase chain reaction device. Photo 3 shows the scientist using an electronic pipette to fill a sample into a micro-reaction tube. Photo 4 shows an Illumina MiSeq sequencer. Photo 5 shows the scientist working on a bioinformatics quality control device. Photo 6 shows the scientist at a desk in front of a computer making inputs for assignment.

Microorganisms, especially fungi and bacteria, play an essential role in soil, including as destructors in the decomposition of organic matter and as symbionts for the growth and health of plant partners. In addition to these interactions with plants, soil microorganisms are influenced by environmental changes such as land use and forest management. However, the degree to which individual factors affect microbial diversity and composition is not yet fully understood.


The main task of the Core Project Microorganisms is the collection and monitoring, especially of the fungal community, on all 300 grassland and forest experimental plots (EPs). In addition to coordinated sampling in May 2011 and 2014, the core project will also be responsible for organising the sampling of all 100 EPs of the Hainich exploratory in 2017.

Furthermore, synthesis activities are taking place between the core project and various projects: e.g., with Norbert Hölzel, University of Münster or Ellen Kandeler and Sven Marhan, University of Hohenheim.


The objectives in the phase (2017-2020) are:

1. Monitoring of soil microorganisms to determine temporal effects of land use and forest management (soil samples from 2011, 2014 and 2017).
2. Record the diversity of arbuscular mycorrhizal fungi (AMF) in all EPs.
3. Study the influence of topsoil disturbance and additional seed placement on the adaptability of soil microorganisms in grasslands (SADE).
4. Analysis of the fungal community under different land use intensity and disturbance within one year (TIMEMIC).


DNA extraction and quantification from soil, creation of a fungal amplicon library and next-generation sequencing with the Illumina MiSeq platform, bioinformatics (MOTHUR & QIIME) and multivariate statistics.


Doc
The influence of forest gaps and their subsequent closure on the diversity of soil fungi
Der Einfluss von Waldlücken auf Bodenpilze
Wöltjen J. (2023): The influence of forest gaps and their subsequent closure on the diversity of soil fungi. Master thesis, University Koblenz-Landau
Doc
Influence of land use on the temporal dynamics of fungal communities across the German Biodiversity Exploratories (BE)
Einfluss von Landnutzung auf die zeitlichen Dynamiken von Bodenpilze in den Deutschen Biodiversitätexploratorien (BE)
Rivera Cerquera D. F. (2023): Influence of land use on the temporal dynamics of fungal communities across the German Biodiversity Exploratories (BE). Bachelor thesis, University of Leipzig
Doc
Goldmann K., Boeddinghaus R. S., Klemmer S., Regan K. M., Heintz-Buschart A., Fischer M., Prati D., Piepho H.-P., Berner D., Marhan S., Kandeler E., Buscot F., Wubet T. (2020): Unraveling spatio‐temporal variability of arbuscular mycorrhiza fungi in a temperate grassland plot. Environmental Microbiology 22 (3), 873-888. doi: 10.1111/1462-2920.14653
More information:  doi.org
Doc
Vergleichbare Pilzgemeinschaften in der Rhizosphäre von ansässigen Pflanzen und Phytometerpflanzen in bewirtschafteten Grünlandökosystemen
Schöps R., Goldmann K., Korell L., Bruelheide H., Wubet T., Buscot F. (2020): Resident and phytometer plants host comparable rhizosphere fungal communities in managed grassland ecosystems. Scientific Reports 10: 919. doi: 10.1038/s41598-020-57760-x
Doc
Goldmann K., Ammerschubert S., Pena R., Polle A., Wu B.-W., Wubet T., Buscot F. (2020): Early stage root-associated fungi show a high temporal turnover, but are independent of beech progeny. Microorganisms 8 (2), 210. doi: 10.3390/microorganisms8020210
More information:  doi.org
Doc
Verbreitung von medizinisch relevanten Antibiotikaresistenzgenen und mobilen genetischen Elementen in Wald- und Grünlandböden
Willms I. M., Yuan J., Penone C., Goldmann K., Vogt J., Wubet T., Schöning I., Schrumpf M., Buscot F., Nacke H. (2020): Distribution of Medically Relevant Antibiotic Resistance Genes and Mobile Genetic Elements in Soils of Temperate Forests and Grasslands Varying in Land Use. Genes 11 (2), 150. doi: 10.3390/genes11020150
More information:  doi.org
Doc
Der Einfluss von Borkenkäfern (Curculionidae, Scolytinae) auf das Mykobiom der Gemeinen Fichte (Picea abies (L.) H. Karst., Pinaceae) im Biodiversitäts-Exploratorium Hainich-Dün
Masch D. (2020): Der Einfluss von Borkenkäfern (Curculionidae, Scolytinae) auf das Mykobiom der Gemeinen Fichte (Picea abies (L.) H. Karst., Pinaceae) im Biodiversitäts-Exploratorium Hainich-Dün. Bachelor thesis, FU Berlin
Doc
Belowground plant microbiome diversity and community composition in grassland ecosystems along land-use gradients in the German Biodiversity Exploratories
Unterirdische Pflanzenmikrobiomvielfalt und Gemeinschaftszusammensetzung in Grünlandökosystemen entlang von Landnutzungsgradienten in den deutschen Biodiversitäts-Exploratorien
Schöps R. (2020): Belowground plant microbiome diversity and community composition in grassland ecosystems along land-use gradients in the German Biodiversity Exploratories. Dissertation, Leipzig University
Doc
Landnutzungsintsität anstatt von funktionellen Pflanzengruppen beeinflussen die bakterielle und pilzliche Rhizosphärengemeinschaft
Schöps R., Goldmann K., Herz K., Lentendu G., Schöning I., Bruelheide H., Wubet T., Buscot F. (2018): Land-use intensity rather than plant functional identity shapes bacterial and fungal rhizosphere communities. Frontiers in Microbiology 9:2711. doi: 10.3389/fmicb.2018.02711
More information:  doi.org
Doc
Auswirkung von pflanzlichen Nachbarschaftseffekten auf die pilzliche Diversität und Gemeinschaftszusammensetzung in der Rhizosphäre
Lohmaier A. (2018): Auswirkung von pflanzlichen Nachbarschaftseffekten auf die pilzliche Diversität und Gemeinschaftszusammensetzung in der Rhizosphäre. Master thesis, University Halle-Wittenberg
Doc
Weißbecker C., Buscot F., Wubet T. (2017): Preservation of nucleic acids by freeze-drying for next generation sequencing analyses of soil microbial communities. Journal of Plant Ecology 10 (1), 81-90. doi: 10.1093/jpe/rtw042
More information:  doi.org

Non-public datasets

Dataset
Forest Gap Experiment (FOX): DNA-based analysis of soil fungal communities (Illumina MiSeq) – ASV abundances
Buscot, Francois; Goldmann, Kezia (2024): Forest Gap Experiment (FOX): DNA-based analysis of soil fungal communities (Illumina MiSeq) - ASV abundances. Version 5. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31838
Dataset
Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2021; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2021; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31955
Dataset
General soil fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) – OTU abundances
Goldmann, Kezia (2024): General soil fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) - OTU abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31960
Dataset
Arbuscular mycorrhizal fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) – AMF taxonomy look-up table
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) - AMF taxonomy look-up table. Version 2. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31959
Dataset
Arbuscular mycorrhizal fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31958
Dataset
Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2023; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2023; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31956
Dataset
Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2014; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2014; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31953
Dataset
Arbuscular mycorrhizal fungi on all 300 EPs (from Soil Sampling Campains 2011, 2014, 2017, 2021 and 2023; Illumina MiSeq) – AMF taxonomic look-up table
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 300 EPs (from Soil Sampling Campains 2011, 2014, 2017, 2021 and 2023; Illumina MiSeq) - AMF taxonomic look-up table. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31957
Dataset
Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2011; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2011; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31952
Dataset
Forest Gap Experiment (FOX): DNA-based analysis of soil fungal communities (Illumina MiSeq) – ASV taxonomic look-up table
Buscot, Francois; Goldmann, Kezia (2024): Forest Gap Experiment (FOX): DNA-based analysis of soil fungal communities (Illumina MiSeq) - ASV taxonomic look-up table. Version 4. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31839
Dataset
Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2023; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2023; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31951
Dataset
Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2017; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 grassland EPs (from Soil Sampling Campain 2017; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31954
Dataset
Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2017; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2017; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31949
Dataset
Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2014; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2014; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31948
Dataset
Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2021; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2021; Illumina MiSeq) - AMF abundances. Version 3. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31950
Dataset
Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2011; Illumina MiSeq) – AMF abundances
Goldmann, Kezia (2024): Arbuscular mycorrhizal fungi on all 150 forest EPs (from Soil Sampling Campain 2011; Illumina MiSeq) - AMF abundances. Version 5. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31947
Dataset
General soil fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) – taxonomy look-up table
Goldmann, Kezia (2024): General soil fungi on agricultural EPs (from soil pilot sampling April 2024; Illumina MiSeq) - taxonomy look-up table. Version 2. Biodiversity Exploratories Information System. Dataset. https://www.bexis.uni-jena.de. Dataset ID= 31961

The so-called core projects of the BE emerged from the site selection project and the establishment of the exploratories (2006-2008). Since 2008, they have been providing the infrastructure and collecting important basic information on land use, diversity and ecosystem processes (long-term monitoring) for all projects. In addition, they coordinate project-wide activities such as various large-scale experiments.

Project in other funding periods

Picture: The collage contains six photographs of steps in molecular analyses in a laboratory, performed by a scientist. Photo 1 shows a sample standardization device. Photo 2 shows the scientist working on a polymerase chain reaction device. Photo 3 shows the scientist using an electronic pipette to fill a sample into a micro-reaction tube. Photo 4 shows an Illumina MiSeq sequencer. Photo 5 shows the scientist working on a bioinformatics quality control device. Photo 6 shows the scientist at a desk in front of a computer making inputs for assignment.
Microorganisms (Core project)
#Microorganisms & Fungi  #BEF  #Soil Ecology  #REX/LUX  #FOX  #2020 – 2023  #Archaea […]
Picture: The collage contains six photographs of steps in molecular analyses in a laboratory, performed by a scientist. Photo 1 shows a sample standardization device. Photo 2 shows the scientist working on a polymerase chain reaction device. Photo 3 shows the scientist using an electronic pipette to fill a sample into a micro-reaction tube. Photo 4 shows an Illumina MiSeq sequencer. Photo 5 shows the scientist working on a bioinformatics quality control device. Photo 6 shows the scientist at a desk in front of a computer making inputs for assignment.
Microorganisms (Core project)
#Microorganisms & Fungi  #BEF  #Soil Ecology  #REX/LUX  #FOX  #2023 – 2026  #Archaea […]

Scientific assistants

Prof. Dr. Francois Buscot (assoz.)
Alumni
Prof. Dr. Francois Buscot (assoz.)
Dr. Tesfaye Wubet
Alumni
Dr. Tesfaye Wubet
Dr. Kezia Goldmann
Employee
Dr. Kezia Goldmann
Helmholtz-Zentrum für Umweltforschung (UFZ)
Ali Nawaz
Alumni
Ali Nawaz
Beatrix Schnabel
Employee
Beatrix Schnabel
Helmholtz-Zentrum für Umweltforschung (UFZ)
Ricardo Schöps
Alumni
Ricardo Schöps
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